The Role of MCT1 in Healthy CNS Physiology, Neurodegenerative Phenomena, and in Facilitating Regeneration in the PNS
MCT1, a pivotal Lactate transporter (along other monocarboxylates such as Pyruvate and β-Hydroxybutyrate among others) has been recently demonstrated as being pivotal for neuronal metabolism, myelin-axon integrity and maintenance as well as facilitation or assistance of axonal regeneration, and neural restoration as consequence. Our lab seeks to study this novel potential mechanisms, studying the central role of MCT1 in a myriad of different applications. We’re currently developing a Conditional MCT1 KO mouse which could be crossed with any inducible Cre models to specifically knock MCT1 out of just about any cell type. The project is still in its juvenescence: ergo, much of our work goes to confirming the knockout model, involving RT-PCR, Western Blots, Immunostaining, Tissue Analysis for signs of developed pathology in neural cells and tissue.
- Brett M. Morrison, Akivaga Tsingalia, Svetlana Vidensky, Youngjin Lee, Lin Jin, Mohamed H. Farah, Sylvain Lengacher, Pierre J. Magistretti, Luc Pellerin & Jeffrey D. Rothstein; Deficiency in monocarboxylate transporter 1 (MCT1) in mice delays regeneration of peripheral nerves following sciatic nerve crush, Experimental Neurology, 263; 2015 Jan: 325-338; DOI information: 10.1016/j.expneurol.2014.10.018
- Youngjin Lee, Brett M. Morrison, Yun Li, Sylvain Lengacher, Mohamed H. Farah, Paul N. Hoffman, Yiting Liu, Akivaga Tsingalia, Lin Jin, Ping-Wu Zhang, Luc Pellerin, Pierre J. Magistretti & Rothstein JD; Oligodendroglia metabolically support axons and contribute to neurodegeneration, Nature, 2012 Jul 11; Nature ID: 113114; PMID: 22801498